Fig.
2. Strand specificity of RT-PCR for HCV RNA using a set
of NT-primer. The upper panel shows no detectable positive strand
HCV RNA in the RT-PCR procedure for n egative strand RNA. The
bottom panel shows no detectable negative strand RNA in the
RT-PCR in the RT-PCR procedure for positive strand RNA. Therefore,
we cl aim that our RT-PCR method is specific and strand specificity
can be maintain ed at a level of 200 ng which is approximately
4.3¡Á10£Û10£Ý HCV RNA molecul es per assay.
