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Year : 2018  |  Volume : 131  |  Issue : 14  |  Page : 1710-1714

Constructing a Novel Three-Dimensional Biomimetic Corneal Endothelium Graft by Culturing Corneal Endothelium Cells on Compressed Collagen Gels

Department of Ophthalmology, Peking University Third Hospital; Beijing Key Laboratory of Restoration of Damaged Ocular Nerve, Peking University Third Hospital, Beijing 100191, China

Correspondence Address:
Dr. Yun Feng
Department of Ophthalmology, Peking University Third Hospital, 49 North Garden Road, Haidian District, 100191 Beijing
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0366-6999.235883

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Background: Endothelium allotransplantation is the primary treatment for corneal decompensation. The worldwide shortage of donor corneal tissue has led to increasing pressure to seek an alternative for surgical restoration of corneal endothelium. Compressed collagen (CC) gels have excellent biocompatibility, simple preparation course and easy to be manipulated. This study aimed to form a new biomimetic endothelium graft by CC. Methods: We expanded bovine corneal endothelial cells (B-CECs) on laminin-coated CC to form a biomimetic endothelium graft. Scanning electron microscope was used for ultrastructural analysis and tight junction protein ZO-1 expression was tested by immunohistochemistry. Results: The biomimetic endothelium graft, we conducted had normal cell morphology, ultrastructure and higher cell density (3612.2 ± 43.4 cells/mm2). ZO-1 localization at B-CECs membrane indicated the bioengineered graft possess the basic endothelium function. Conclusions: A biomimetic endothelium graft with B-CECs expanded on CC sheet was constructed, which possessed cells' morphology similar to that of in vivo endothelial cells and specific basic function of endothelium layer. This method provided the possibility of using one donor's cornea to form multiple uniformed endothelium grafts so as to overcome the shortage of cadaveric cornea tissue.


 Abstract in Chinese





结果:在压缩胶原薄片上制备出单层且具有正常内皮细胞六角形态、超微结构和高细胞密度(3612.2 ± 43.4 cells/mm2)的角膜内皮植片,ZO-1正常表达于植片内皮细胞的细胞膜上。


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