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Year : 2018  |  Volume : 131  |  Issue : 14  |  Page : 1694-1701

Effect of Dandelion Extracts on the Proliferation of Ovarian Granulosa Cells and Expression of Hormone Receptors

1 Department of Reproductive Medicine, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China
2 Core Facility Center, Capital Medical University, Beijing 100069, China
3 Hanabusa Women's Clinic, Tokujun Company, 4-2-15 Gokodori Chuo-Ku, Kobe, Hyogo, Japan

Correspondence Address:
Dr. Yan-Min Ma
Department of Reproductive Medicine, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0366-6999.235864

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Background: In the current society, infertility related to age has become a social problem. The in vitro fertilization (IVF) success rate in women with poor ovarian response (POR) is very low. Dandelion extract T-1 (DE-T1) is an effective component of the extract from the leaves and stems of Taraxacum officinale, which is one of the medicines used in some patients with POR, but its molecular mechanism remains unclear. Methods: Following IVF, ovarian granulosa cells (GCs) of sixty patients were extracted and divided into normal ovarian response (NOR) and POR groups. GCs were cultured in a dose-dependent and time-dependent manner with DE-T1, proliferation of GCs was determined by Cell Counting Kit-8 assay, and mRNA levels of insulin-like growth factor 1 receptor (IGF-1R), luteotropic hormone receptor (LHR), follicle-stimulating hormone receptor (FSHR), LHR, and CYP19A1 (aromatase) were determined by quantitative polymerase chain reaction. Progesterone and estradiol (E2) concentrations were determined by enzyme-linked immunosorbent assay. Results: The cell viability gradually increased with the progressive increase in the DE-T1 concentration. Compared with the control group (without DE-T1), the mRNA expressions of FSHR, LHR, IGF-1R, and CYP19A1 were upregulated after the addition of DE-T1, especially in the 2.5% DE-T1 group (P < 0.01). The expression of IGF-1R was upregulated approximately 25 times (24.97 ± 4.02 times) in the POR group with 2.5% DE-T1. E2 and progesterone levels increased with the increasing DE-T1 concentration. There were highly significant differences in the E2 and progesterone secretion between the NOR and POR groups (P < 0.01). Conclusion: DE-T1 may promote steroid hormone synthesis by promoting GC proliferation and upregulating GC receptor expression, thereby improving ovarian endocrine function.


 Abstract in Chinese




方法: 收集行体外受精-胚胎移植(in vitro fertilization -embryo transfer,IVF-ET)卵巢低反应组(poor ovary response,POR)和卵巢正常反应组(normal ovary response,NOR)患者各30例的卵泡液,并提取颗粒细胞,原代培养24 h后分别在含DE-T1低(1.25%)、中(2.5%)、高(5%)剂量的培养液内培养孵育48~60h,采用CCK-8法检测颗粒细胞活性。荧光定量PCR方法检测颗粒细胞胰岛素样生长因子1受体(insulin-like growth factor-1 receptor,IGF1-R)、促卵泡激素受体(follicle-stimulating hormone receptor, FSHR)、黄体生成素受体(luteinizing hormone,LHR)和CYP19A1mRNA的表达。采用酶联免疫吸附法(ELISA)测定颗粒细胞培养液中孕酮(progesterone,P)、雌二醇(Estradiol,E2)浓度。


结论:蒲公英提取物DE-T1可以促进颗粒细胞的增殖,促进FSHR、LHR、IGF-1R、CYP-19A1 mRNA的表达,促进卵巢颗粒细胞类固醇激素的合成。

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